The Wein Lab is focusing on Neuromuscular Disorders such as Duchenne Muscular dystrophy, which is a devastating disease leading to death of patients at age 20-30 years. Our lab developed an expertise in translational research, an emerging field which can be summarized as understanding the cause of a disease and learning from it in order to develop innovative therapeutic strategies. We are fully committed to continuing to integrate basic science and gene therapy skills in order to advance understanding and treatment of the muscular dystrophies. Our lab's long term projects focus on RNA and DNA editing and gene transfer as therapies for neuromuscular disorders, as well as furthering fundamental research on muscle function. In particular, our lab uses the most recent technologies such as virus-mediated exon-skipping and the CRISPR/Cas9 genome editing tool.

We are looking for a highly motivated research associate who is energetic and intelligent to join our dynamic team. In particular, we are looking for someone with expertise in molecular biology (qPCR, IF, WB), as well as handling mouse colonies, mouse injection and dissection experiences to collect all muscles and peripheral organs. Experience in mounting muscle tissue is preferred. No background in gene therapy is required, however the candidate should have a high interest in human genetics and diseases and should be eager to learn and work on the bench in an independent manner. Finally, our team relies on good teamwork and therefore, the candidate should be a good communicator and team player. The job description includes, but is not restricted to performing research experiments, maintaining records of experiments, preparing laboratory reports, analyzing data and preparing publications and grants applications.

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Performs research experiments semi-independently or independently after training in accordance with protocols approved by the principal investigator. Such experiments may involve the use of laboratory animals or human subjects, any may require evening and/or weekend duties.
• Handle and manage mouse colonies and performed behavior tests and injections. Prepares and tests substrate, conducts assays (ddPCR, qPCR, RT-PCR, Western Blot, Immunofluorescence), and prepares tissue cultures for use in research studies, as applicable. Collects muscle, blood and other samples as needed. Such activities may be performed independently and may require evening and weekend hours.
• Ensures accuracy of laboratory analytical procedures as required by specific research projects.
• Collects and controls experimental data; and maintains accurate daily logs of experiments including methods, results, and conclusions.
• Interprets the results of data analyses with input from the principal investigator.
• Assists the principal investigator in the preparation of scientific publications and grant proposals.
• Make recommendations about refinement and/or replacement of laboratory analytical procedures. Implements such refinements or new procedures with input from the principal investigator.
• Orders supplies needed to conduct research; maintains inventory of equipment and supplies; and assures that research equipment is in good working order.
• Collaborates in the preparation of research budgets, and monitors expenditure of funds to assure that studies are conducted within budgetary constraints.
• Instructs other laboratory workers and students in standard methods, practices, procedures, and equipment operations.
• Maintains a clean working area and performs general laboratory maintenance. Adheres to proper safety techniques and procedures.
• Performs miscellaneous related duties.

KNOWLEDGE , SKILLS AND ABILITIES REQUIRED

• Bachelor of Science degree with four to five year of laboratory research experience.Laboratory experience is requiredCoursework in dissection, muscle physiology and relevant research methods are desirable

Laboratory experience in the following is required:

• Handling, managing mouse colonies and mouse dissection.

Laboratory experience in the following is preferred:

• Performing behavior tests and injections. Preparing and conducting assays (ddPCR, qPCR, RT-PCR, Western Blot, Immunofluorescence). Preparing tissue cultures for use in research studies, as applicable. Collects muscles, peripheral organs, blood and other samples as needed as well as cryosectioning those tissues.

Minimum Physical Requirements:

• Percent of time spent on: 50% Preparing test substrates, conducting assays, preparing cultures25% Interpreting results of data analyses with PI/postdoctoral students.11% Miscellaneous paperwork associated with research10% Ordering supplies02% Preparation of budget02% Instructing other lab personnel
• 100% Kneeling / reaching100% Finger dexterity100% Talking / hearing / seeing 33% Lifting / carrying weight 0-20 pounds for 1-5 minutes
• The above list of duties is intended to describe the general nature and level of work performed by individuals assigned to this classification. It is not to be construed as an exhaustive list of duties performed by the individual so classified, nor is it intended to limit or modify the right of any supervisor to assign, direct, and control the work of employees under his/her supervision.
• EOE M/F/Disability/Vet