The Johns Hopkins University department of Genetic Medicine is seeking a Research Technologist who will be responsible for sample handling, planning and executing molecular genetics tests (i.e., genotyping or sequencing) on large numbers of DNA samples.

Specific Duties & Responsibilities

• Transfer DNA samples into 96-well plate format.
• Receive and prepare Deepwell DNA stock plates and create working DNA plates.
• Determine concentration and volume of DNA samples using various instruments.
• Dilute samples to working concentrations.
• Maintain and monitor inventory of all in-house DNA samples and reagent supplies.
• Create master mixes; Set up polymerase chain reactions; trouble-shoot such reactions.
• Operate and troubleshoot robotic liquid handlers.
• Prepare and complete SNP genotyping assays including, but not limited to, Illumina assays and workflows.
• Prepare samples for targeted capture of specific regions of DNA for sequencing experiments. This includes library preparation and capture techniques.
• Prepare and complete Next-Gen sequencing assays including, but not limited to, Illumina NovaSeq workflows.
• Perform quality control checks of data using various software, including, but not limited to, GenomeStudio, Spotfire and other in-house QC metrics reports.
• Maintain timely and accurate laboratory records (electronic and written).
• Collect, organize, interpret and submit experimental data to database and supervisors.
• Perform general lab maintenance duties.
• Perform validation tests of lab reagents.
• Participate in committees that function to improve processes.
• Communicate effectively to supervisor and team members.
• Perform miscellaneous related duties as assigned.

Special Knowledge, Skills, & Abilities

• Basic understanding of molecular biology and genetics.
• Requires the ability to safely operate scientific laboratory equipment.
• Use of special equipment, machines and computer(s): Centrifuges, Illumina BeadLab system (Tecans, iScans, HiScans, TeFlow), Covaris, BioAnalyzer, Nanodrop instruments, Illumina NovaSeq, SpriWorks, thermal cyclers, robotic and manual liquid handlers, common laboratory equipment, Macintosh, PC computers, UNIX servers, Oracle database.
  

• The job requires superior organizational skills for time and data management. Ability to function well in a team production setting is necessary.
  

• Excellent communication (spoken word) skills.
• Position will require flexibility to work hours beginning as early as 6:00 a.m. and/or ending as late as 6:00 p.m.

Physical Requirements

• Remain in seated position for extended periods of time.
• Reaching by extending hand(s) or arm(s) in any direction.
• Finger dexterity to manipulate objects with fingers rather than with whole hand or arm, for example, using a keyboard.
• Ability to see and hear within normal parameters; Ability to move about workspace. Lift, carry, move supplies, computers, etc.

• Bachelor's Degree in Biology, Chemistry or related field.

• Some related post-undergraduate work experience.

Classified Title: Research Technologist
Role/Level/Range: ACRO40/E/02/CC
Starting Salary Range: $15.50 - $26.00 HRLY ($43,160 targeted; Commensurate with experience)
Employee group: Full Time
Schedule: M-F, 8:30 to 5pm
Exempt Status:Non-Exempt
Location:School of Medicine Campus
Department name: SOM Genetics Ctr for Inhrtd Dis Res CIDR
Personnel area: School of Medicine