The Johns Hopkins University is seeking aResearch Specialist II who will perform laboratory procedures, data processing, and laboratory maintenance following common or established protocols and procedures.

The Single Cell & Transcriptomics Core serves the Johns Hopkins Community as well as the world-wide scientific community. The Core provides cost effective Single Cell and Next Generation sequencing analysis enabling more researchers to access advanced transcriptomics and genomics services. The Core is a fast paced and growing lab that requires additional technicians to aid in sample processing and tracking, lab organization, primary data analysis.

Specific Duties & Responsibilities

• Under the general supervision of a Core Director or Laboratory Supervisor, the Specialist is responsible for performing quality control analysis on client and Core generated RNA, DNA and library samples and returning data to clients.
• Prepare libraries for and load, 10x Chromium, Mission Bio Tapestri, Next Generation sequencers or any newly acquired instrument.
• Perform RNA and DNA extractions and advanced molecular biology sample processing.
• Analyze raw sequencing data within Illumina BaseSpace software.
• Analyze raw sequencing data for 10x methods, cell or tissue based.
• Trains new hires on SOPs, operations, and maintenance of equipment in the core.
• Perform general lab maintenance, including unpacking orders, signing in projects, and updating sample tracking software.
• Perform routine maintenance on all instruments, such as cleaning/washing, calibration/system check, software updates, and power cycles.
• Consults industry tech support to replace kits whenever something fails unexplainably and to troubleshoot what may have caused the problem and how to avoid it in the future.
• Collaborate with clients/researchers to understand their desired project requirements and deadlines and to provide technical guidance on sample preparation for sequencing.
• Explain any relevant methods to clients so that they can better understand the technology.
• Communicate sequencing results and basic data analysis to researchers in a clear and concise manner.
• Maintain and update sample submission records.
• Help develop and implement standard operating procedures and best practices for the core facility.
• Oversee multiple projects simultaneously, ensuring that deadlines are met, and quality standards are maintained.
• Establish a collaborative learning environment within the lab, promoting continuous skill development and knowledge sharing.
• Manage inventory, monitors supply levels, orders equipment, and supplies in accordance with established policies and procedures, and initiate the process for equipment repair when needed.
• Teach/train students, and new technicians in the lab. Guide, direct, and facilitate problem solving for entry-level lab members on day-to-day problems.
• Understand and use software packages used by SCTC for data analysis.
• Adhere to all SCTC Core requirements.
• Perform miscellaneous related duties as assigned.

• Bachelor's Degree in Biology, Chemistry or related field.
• Two years laboratory experience performing relevant techniques.
• Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.

• Chromium system.
• Hands-on experience with 10x.

Classified Title: Research Specialist II
Role/Level/Range: ACRP/03/MB
Starting Salary Range: $40,900 - $71,600 Annually ($60,000 targeted; Commensurate with experience)
Employee group: Full Time
Schedule: M-F 8:30-5:00
Exempt Status:Exempt
Location:School of Medicine Campus
Department name: SOM Bas Sci Inst Service Centers
Personnel area: School of Medicine